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產(chǎn)品簡介

Chicken TNF-α ELISA Kit

Chicken TNF-α ELISA Kit

價格:電議

采購度:787    原產(chǎn)地:美洲

發(fā)布時間:2012/1/30 15:49:41

Chicken TNF-α ELISA Kit For the quantitative in vitro determination of Chicken Tumor necrosis factor α concentrations in serum - plasma - celiac fluid - tissue homogenate - body fluid

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標(biāo)簽:Chicken   TNF-α   ELISA   Kit   

詳細(xì)信息
INTENDED USE AND TEST PRINCIPLE
This TNF-α ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of TNF-α in the sample, this TNF-α ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus TNF-α concentration. The concentration of TNF-α in the samples is then determined by comparing the O.D. of the samples to the standard curve.

SAMPLE COLLECTION AND STORAGES
Serum - Use a serum separator tube and allow samples to clot for 2 hours at room temperature or overnight at 4℃ before centrifugation for 20 minutes at approximately 2000×g. Remove serum and assay immediately or aliquot and store samples at -20℃. Avoid repeated freeze-thaw cycles
Plasma - Collect plasma using heparin as an anticoagulant. Centrifuge samples for 30 minutes at 2000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃. Avoid repeated freeze-thaw cycles.
Tissue homogenates - For general information, hemolysis blood may affect the result, so you should rinse the tissues with ice-cold PBS (0.01M, pH=7.4) to remove excess blood thoroughly. Tissue pieces should be weighed and then minced to small pieces which will be homogenized in PBS (the volume depends on the weight of the tissue. 9mL PBS would be appropriate to 1 gram tissue pieces. Some protease inhibitor is recommended to add into the PBS.) with a glass homogenizer on ice. To further break the cells, you can sonicate the suspension with an ultrasonic cell disrupter or subject it to freeze-thaw cycles. The homogenates are then centrifugated for 5minutes at 5000×g to get the supernate.
Cell culture supernates, tissue homogenate and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃. Avoid repeated freeze-thaw cycles.
Note: The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.

CALCULATION OF RESULTS
1.This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (X) axis versus the corresponding concentration on the horizontal (Y) axis.
2.First, calculate the mean O.D. value for each standard and sample. All O.D. Values are subtracted by the mean value of the balnk well before result interpretation. Construct the standard curve using graph paper or statistical software.
3.To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration.
4.Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.
5.Intra-assay CV(%) is less than 10% and Inter-assay CV(%) is less than 15%.
6.Assay range: 2.5 pg/mL – 80 pg/mL.
7. Sensitivity: The minimum detectable dose of Chicken TNF-α is typically less than 0.1 pg/mL.
8. Cross-reactivity: This assay recognizes recombinant and natural Chicken TNF-α. No significant cross-reactivity or interference was observed.
9. Storage: 2-8℃ (Use frequently); six months (-20℃)。
10. Standard curve

更新時間:2024/12/23 9:36:23

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